Type
Colorimetricassay
Applications
Cellcultureand/oranimalstudies,Tissue
Storage/Expiration
Allcomponentsarestableforoneyear,(fromInvoiceDate),whenstoredat15–25°C.Theglassvialcontainingcollagenstandardshouldbestoredat+4°Conceopened.
CalibrationRange
Solublecollagen:
2.5 to12.5 μgusing250μlofAlkaliReagenttorecoverthecollagenbounddye
10 to50 μgusing1000μlofAlkaliReagenttorecoverthecollagenbounddye
Insolublecollagen:20 –60 μgusingdenaturedcollagenstandards
LimitofDetection
Solublecollagen:1 μg
Insolublecollagen:10 μg
