Type
Recombinant
Description
TNF-αisapleiotropicpro-inflammatorycytokinesecretedbyvariouscellsincludingADIpocytes,activatedmonocytes,macrophages,Bcells,Tcellsandfibroblasts.ItbelongstotheTNFfamilyofligandsandsignalsthroughtworeceptors,TNFR1andTNFR2.TNF-αiscytotoxictoawidevarietyoftumorcellsandisanessentialfactorinmediatingtheimmuneresponseagainstbacterialinfections.TNF-αalsoplaysaroleintheinductionofsepticshock,autoimmunediseases,rheumatoidarthritis,inflammation,anddiabetes.RecombinantmurineTNF-αisasoluble156 aminoacidprotein(17.3 kDa)whichcorrespondstoC-terminalextracellulardomainofthefulllengthtransmembraneprotein.
AminoAcidSequence
MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50asdeterminedbythecytolysisofmurineL929cellsinthepresenceofactinomycinDis≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
