Type

Recombinant

Description

TIMP-2 isanextracellularinhibitorofMMPs,includingMMP-1, –2, –3, –7, –8, –9, –10, –12, –13, –14, –15, –16and –19.ItbelongstotheI35(TIMP)familyofirreversIBLeproteaseinhibitorsthatfunctionaskeymodulatorsofextracellularmatrixdegradationduringtissuedevelopmentandremodeling.TIMP-2 canalsoactthroughanMMP-independentmechanisminhibitingendothelialcellproliferationinvitroanddemonstratesanti-angiogenicactivitiesinvivo.RecombinantHumanTIMP-2 isa21.8 kDaproteincontaining194 aminoacidresidues.

AminoAcidSequence

CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

Source

E.coli

Purity

95%

BIOLOGicalActivity

TIMP-2 activitywasmeasuredbyitsABIlitytoinhibithumanMMP-1 inducedhydrolysisofachromogenicpeptidesubstrateatroomtemperature.HalfmaximalinhibitionwasobtainedataTIMP-2 concentrationofapproximately0.5 µg/ml,whenusinganMMP-1 concentrationof1.6 µg/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C