Type
Recombinant
Description
CD40,amemberoftheTNFreceptorfamily,isacellsurfaceproteinexpressedonBcells,dendriticcells,monocytes,thymicepithelialcellsand,atlowlevels,onTcells.SignalingthoughCD40playsanimportantroleintheproliferationanddifferentiationofBcells,andiscriticalforimmunoglobulin(Ig)classswitching.Themembrane-anchoredCD40-LigandisexpressedalmostexclusivelyonactivatedCD4+Tlymphocytes.FailuretoexpressCD40Lleadsto“immunodeficiencywithhyper-IgM”,adiseasecharacterizedbyfailuretoproduceIgG,IgAandIgE.ThesolubleformofCD40Lisan18kDaproteincomprisingtheentireTNFhomologousregionofCD40LandisgeneratedinvivobyanintracellularproteolyticprocessingofthefulllengthCD40L.RecombinantMurineCD40Lisasoluble16.4 kDaproteincontaining149 aminoacidresiduescomprisingthereceptorbindingTNF-likedomainof CD40L.
AminoAcidSequence
MQRGDEDPQIAAHVVSEANSNAASVLQWAKKGYYTMKSNLVMLENGKQLTVKREGLYYVYTQVTFCSNREPSSQRPFIVGLWLKPSSGSERILLKAANTHSSSQLCEQQSVHLGGVFELQAGASVFVNVTEASQVIHRVGFSSFGLLKL
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinduceTNF-αandMIP-1αproductionbymurinesplenocytes.TheexpectedED50forthiseffectis<0.1 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
