Type
Recombinant
Description
TheR-Spondin(Rspo)proteinsbelongtotheRspofamilyofWntmodulators.Currently,thefamilyconsistsoffourstructurally-related,secretedligands(Rspo1–4),allcontainingfurin-likeandthrombospondinstructuraldomains.TheRspoproteinscaninteractwiththeFrizzled/LRP6receptorcomplexinamannerthatcausesthestABIlization,andresultingaccumulation,oftheintracellularsignalingprotein,β-catenin.Thisactivityeffectivelyactivatesandincreasesthesubsequentnuclearsignalingofβ-catenin.R-SpondincanalsobindtothepreviouslydiscoveredG-proteincoupledreceptors,LGR-4 andLGR-5. Rspo/β-cateninsignalingcanactasaninducerofthetransformedphenotype,andcanalsoregulatetheproliferationanddifferentiationofcertainstemcellpopulations.RecombinantHumanR-Spondin-2 isa24.4 kDaproteinconsistingof212 aminoacidresidues.Duetoglycosylation,R-Spondin-2 migratesatanapparentmolecularweightofapproximately30.0 kDabySDSPAGEanalysisunderreducingconditions.
AminoAcidSequence
ASYVSNPICKGCLSCSKDNGCSRCQQKLFFFLRREGMRQYGECLHSCPSGYYGHRAPDMNRCARCRIENCDSCFSKDFCTKCKVGFYLHRGRCFDECPDGFAPLEETMECVEGCEVGHWSEWGTCSRNNRTCGFKWGLETRTRQIVKKPVKDTILCPTIAESRRCKMTMRHCPGGKRTPKAKEKRNKKKKRKLIERAQEQHSVFLATDRANQ
Source
ChineseHamsterOvaryCells(CHO)
Purity
95%
BIOLOGicalActivity
R-Spondin-2 enhancesBMP-2-mediateddifferentiationofMC3T3-E1cells.TheexpectedED50forthiseffectis0.8 –2.0 μg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–0.5 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
