Type
Recombinant
Description
Total149 AA.MW:17.36 kDa(calculated).UniProtKBacc.no.Q9UHV8.N-TerminalHis-tag(10 extra AA)
AminoAcidSequence
MKHHHHHHASMSSLPVPYKLPVSLSVGSCVIIKGTPIHSFINDPQLQVDFYTDMDEDSDIAFRFRVHFGNHVVMNRREFGIWMLEETTDYVPFEDGKQFELCIYVHYNEYEIKVNGIRIYGFVHRIPPSFVKMVQVSRDISLTSVCVCN
Source
E.coli
Purity
>90%
SDS-PAGEGel
14%SDS-PAGEseparationofHumanPP13
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,5μg/lane
3.non-reducedandnon-boiledsample,5μg/lane
Formulation
Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin0.05 Mphosphatebuffer,0.075 MNaCl,pH 7.4
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting,ELISA,Immunoassays
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.