Type

Recombinant

Description

LIGHTbelongstotheTNFfamilyofligands,andcansignalthroughtheherpesvirusentrymediatortypeA receptor(HVEM,TNFRSF14),LTβR,orbindtoadecoyreceptor,DcR3.Itisexpressedinsplenocytes,activatedPBL,CD8+tumorinfiltratinglymphocytes,granulocytes,andmonocytes.LIGHThastheABIlitytoactiveNF-κB,toco-stimulatetheactivationoflymphocytesandtoinduceapoptosisincertainhumantumorcells.RecombinantMurineLIGHTisa20.1 kDaproteincontaining183 aminoacidresidues,comprisingtheTNF-homologousregionoftheLIGHTextracellulardomain.

AminoAcidSequence

MRLHQRLGDIVAHLPDGGKGSWEKLIQDQRSHQANPAAHLTGANASLIGIGGPLLWETRLGLAFLRGLTYHDGALVTMEPGYYYVYSKVQLSGVGCPQGLANGLPITHGLYKRTSRYPKELELLVSRRSPCGRANSSRVWWDSSFLGGVVHLEAGEEVVVRVPGNRLVRPRDGTRSYFGAFMV

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitscytotoxiceffectonHT-29 cells(humancolonadenocarcinomacells)inthepresenceofhuman IFN-γ.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C