Type
Recombinant
Description
I-TACisa“non-ELR”CXCchemokinethatisregulatedbyinterferonandsignalsthroughtheCXCR3receptor.I-TACischemoattractantforIL-2-activatedTcells,butdoesnotaffectfreshlyisolatedun-stimulatedTcells,neutrophils,ormonocytes.RecombinantMurineI-TACisa9.0 kDaproteincontaining79 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCXCchemokines.
AminoAcidSequence
FLMFKQGRCLCIGPGMKAVKMAEIEKASVIYPSNGCDKVEVIVTMKAHKRQRCLDPRSKQARLIMQAIEKKNFLRRQNM
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytochemoattractmurineCXCR3-transfectedHEK293cellsusingaconcentrationrangeof10.0–100.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C