Type
Recombinant
Description
HB-EGFisanEGF-relatedgrowthfactorthatsignalsthroughtheEGFreceptor,andstimulatestheproliferationofsmoothmusclecells(SMC),fibroblasts,epithelialcells,andkeratinocytes.HB-EGFisexpressedinnumerouscelltypesandtissues,includingvascularendothelialcells,andvascularSMC,macrophages,skeletalmuscle,keratinocytes,andcertaintumorcells.TheABIlityofHB-EGFtospecificallybindheparinandheparinsulfateproteoglycansisdistinctfromotherEGF-likemolecules,andmayberelatedtotheenhancedmitogenicactivity,relativetoEGF,thatHB-EGFexertsonsmoothmusclecells.ThehumanHB-EGFgeneencodesa208 aminoacidtransmembraneprotein,whichcanbeproteolyticallycleavedtoproducesolubleHB-EGF.RecombinantHumanHB-EGFisa9.7 kDaproteincontaining86 aminoacidresidues,correspondingtotheextracellularEGF-likeandheparin-bindingdomainsofthefulllengthHB-EGFprotein.
AminoAcidSequence
DLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSL
Source
E.coli
Purity
95%
BIOLOGicalActivity
TheED50 wasdeterminedbyacellproliferationassayusingbalb/c3T3cellsis1.0 ng/ml,correspondingtoaspecificactivityof1×106 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
