Type
Recombinant
Description
G-CSFisahematopoieticgrowthfactorthatstimulatesthedevelopmentofcommittedProgenitorcellstoneutrophilsandenhancesthefunctionalactivitiesofthematureend-cell.Itisproducedinresponsetospecificstimulationbyavarietyofcells,includingmacrophages,fibroblasts,endothelialcellsandbonemarrowstroma.G-CSFisbeingusedclinicallytofacilitatehematopoieticrecoveryafterbonemarrowtransplantation.HumanandmurineG-CSFiscross-speciesreactive.RecombinantMurineG-CSFisa19.0 kDaproteinconsistingof179 aminoacidresidues.
AminoAcidSequence
MVPLVTVSALPPSLPLPRSFLLKSLEQVRKIQASGSVLLEQLCATYKLCHPEELVLLGHSLGIPKASLSGCSSQALQQTQCLSQLHSGLCLYQGLLQALSGISPALAPTLDLLQLDVANFATTIWQQMENLGVAPTVQPTQSAMPAFTSAFQRRAGGVLAISYLQGFLETARLALHHLA
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofmurineNFS-60 cellsis≤0.05 ng/ml,correspondingtoaspecificactivityof≥2×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
