Type

Recombinant

Description

Endostatinisanaturallyoccurring20 kDapolypeptidederivedfromtheC-terminalportionoftypeXVIIIcollagen.Itfunctionsasananti-angiogeniccytokinethatisexpressedinvariousorgans,withthehighestlevelsinliver,lungandkidney.Endostatininhibitsangiogenesisbyblockingthepro-angiogenicactivitiesofVEGFandFGF-basic.RecombinantHumanEndostatinisa20.2 kDaproteinconsistingof184 aminoacidresidues.

AminoAcidSequence

MHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK

Source

E.coli

Purity

98%

BIOLOGicalActivity

MeasuredbyitsABIlitytoinhibitHUVECmigration.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C