Type

Recombinant

Description

TheoriginallydescribedIL-17 protein,nowknownasIL-17A,isahomodimeroftwo136 aminoacidchainsthataresecretedbyactivatedT-cells,whichactonstromalcellstoinduceproductionofproinflammatoryandhematopoieticbioactivemolecules.Today,IL-17 representsafamilyofstructurally-relatedcytokinesthatshareahighlyconservedC-terminalregion,butdifferfromoneanotherintheirN-terminalregionsandintheirdistinctBIOLOGicalroles.Thesixknownmembersofthisfamily,IL-17AthroughIL-17F,aresecretedashomodimers.IL-17Aexhibitscross-speciesbioactivitybetweenhumanandmurinecells.RecombinantHumanIL-17Aisa31.3 kDa,disulfide-linkedhomodimeroftwo137 aminoacid,polypeptidechains.

AminoAcidSequence

MIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA

Source

E.coli

Purity

98%

BiologicalActivity

MeasuredbyitsABIlitytoinduceIL-6 productionbyNHDFcellsusingaconcentrationrangeof0.5–1.5 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C