Type

SandwichELISA,Biotin-labelledantibody

Applications

Serum,Plasma-EDTA,Plasma-Heparin,Plasma-Citrate

SampleRequirements

7 µl/well

Storage/Expiration

Storethekitat2–8°C.Undertheseconditions,thekitisstableuntiltheexpirationdate(seelabelonthe box).

CalibrationCurve

CalibrationRange

100–4000pg/ml

LimitofDetection

AnalyticalLimitofDetectioniscalculatedfromtherealleptinvaluesinwellsandis30 pg/mlformouseleptinand50 pg/mlforrat leptin

Intra-assay(Within-Run)

n=8;CV=2.2%

Inter-assay(Run-to-Run)

n=8;CV=3.4%

SpikingRecovery

94,50%

DilutationLinearity

96,70%

Crossreactivity